Rice blackstreaked dwarf virus p10 suppresses protein. Distribution and genetic diversity of southern rice black. Srbsdv has recently spread throughout southern china and vietnam 5,6. The p71 protein of southern rice blackstreaked dwarf virus, a fijivirus, induces the formation of tubular structures in insect cells.
This virus, which was first found in 2001 in guangdong province, china, has in recent years spread rapidly throughout southern china and northern vietnam and poses a. Effects of southern rice blackstreaked dwarf virus on the. Read proteomic analysis of interaction between p71 of southern rice blackstreaked dwarf virus and the insect vector reveals diverse insect proteins involved in successful transmission, journal of proteomics on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. May 17, 2012 transgenic rice oryza sativa plants expressing the pns11 protein of rice gall dwarf virus rgdv displayed multiple abnormal phenotypes, some of which were highly reminiscent of the symptoms observed in rgdvinfected rice. To well understand the destructive effects of rbsdv infection on maize growth, comparative proteomic. Southern rice black streaked dwarf virus srbsdv and rice black streaked dwarf virus rbsdv are serious riceinfecting reoviruses, which are transmitted by different planthoppers in a persistent. Southern rice blackstreaked dwarf virus srbsdv directly. Relation between the viral load accumulation of southern. This virus is difficult to diagnose in the early stages of infection, and is very destructive at the late stage. Southern rice blackstreaked dwarf virus srbsdv transmitted by the whitebacked planthopper wbph, sogatella furcifera horvath, is a recently identified member of the genus fijivirus in the family reoviridae. Srbsdv has morphological and serological similarities to rice blackstreaked dwarf virus rbsdv, a recognized member of the genus fijivirus, but.
Plant protection station, agricultural department of hainan province, haikou. Rice and maize dwarf diseases caused by the newly introduced southern rice black streaked. Southern rice blackstreaked dwarf virus srbsdv, a genus fijivirus of the family reoviridae, is transmitted by sogatella furcifera whitebacked planthopper, wbph. Knowledge bank rice blackstreaked dwarf virus plantwise. Southern rice blackstreaked dwarf disease srbsdd was. In this study, we conducted a genomewide association study gwas on rbsdv.
This pest attacks rice, leading to yellowing or hopper burn, which causes the plants to wilt and can damage the grains. Analysis of sogatella furcifera proteome that interact. Southern rice blackstreaked dwarf virus srbsdv or rice blackstreaked dwarf virus 2 was first reported in china and was identified as a novel putative member of the genus fijivirus within the family reoviridae 1 5. Therefore, determining rice cultivars with high resistance to srbsdv and rrsv is necessary. The virus is propagatively transmitted by the planthopper, laodelphax striatellus fallen, to rice plants in a persistent manner ruan et al, 1984. Southern rice blackstreaked dwarf virus srbsdv was a new pathogen species in genus group fijivirus 2, which caused serious yield loss of many important cereal crops, such as. Rice is often subjected to biotic stressors, such as fungi, bacteria, and viruses 1. The complete genome sequence of two isolates of southern rice black. Overexpression of rice blackstreaked dwarf virus p71 in.
Rna silencing suppressor pns11 of rice gall dwarf virus. These two viruses are members of the genus fijivirus in the family reoviridae and can cause similar dwarf symptoms in rice. Southern rice blackstreaked dwarf virus srbsdv threatens rice production in china and. This virus, which was first found in 2001 in guangdong province, china, has in recent years spread rapidly throughout southern china and northern vietnam and poses a great threat to rice production in these areas li et. Maize rough dwarf disease mrdd is a severe disease that has been occurring frequently in southern china and many other asian countries. Southern rice blackstreaked dwarf virus srbsdv is a novel viral species in the genus fijivirus, family reoviridae, proposed by zhang et al. This first complete genomic sequence of mrdv will help understand the phylogenetic relationships among group 2 fijiviruses and especially the closely related rice blackstreaked dwarf virus, which is also found to naturally infect maize. Rice, as an important crops in worldwide, is a primary food source for half of the worlds population. Unfortunately, studies on effective antiviral drugs against srbsdv and interaction mechanism of antiviral molecule targeting on srbsdv have not been reported.
Sep 22, 2016 southern rice black streaked dwarf virus srbsdv, recognized as a new member of the genus fijivirus in the family reoviridae was first discovered in guangdong province, china in 2001 1. Control measures based on protection from wbph, including seedbed. Southern rice black streaked dwarf virus srbsdv and rice black streaked dwarf virus rbsdv are serious rice infecting reoviruses, which are transmitted by different planthoppers in a persistent. All lower taxonomy nodes 1 common name isynonym iother names i rice southern black streaked dwarf virus southern rice black streaked dwarf virus. The rice blackstreaked dwarf virus rbsdv disease causes severe rice yield losses in eastern china and other east asian countries. Hostplant resistance an overview of the control strategies for rbsdv and other rice viruses is given by ishii 1973 and abo and sy 1998. It is proposed that this virus be considered as a new species, southern rice blackstreaked dwarf virus, in the group 2 of. Southern rice black streaked dwarf virus srbsdv is a novel viral species in the genus fijivirus, family reoviridae, proposed by zhang et al. Rice black streaked dwarf virus rbsdv and southern rice black streaked dwarf virus srbsdv seriously interfered in the production of rice and maize in china. Delphacidae is an economically important pest, which causes severe damages to rice production in east asian due to transmission of rice stripe virus rsv and rice black. Close evolutionary relationship between rice black. Analysis of sogatella furcifera proteome that interact with. Identification of new rice cultivars and resistance loci. Large numbers of small galls appear about 20 days after inoculation in rice infected with rice gall dwarf virus putta et al.
Detection, occurrence, and survey of rice stripe and black. Accumulation of southern rice black streaked dwarf virus srbsdv and rice black streaked dwarf virus rbsdv genome equivalent copies gec in the hemolymph of the vector and nonvector insect species over time using rtqpcr a and conventional rtpcr for srbsdv 620bp band b and rbsdv 969bp band c. Jan 24, 2020 the p71 protein of southern rice blackstreaked dwarf virus, a fijivirus, induces the formation of tubular structures in insect cells. Evaluation of rice resistance to southern rice black. Fijivirus article about fijivirus by the free dictionary. In host range and symptomatology, properties in vitro and transmission by planthoppers, rice black streaked dwarf virus closely resembles maize rough dwarf virus, and luisoni et al. The distribution and diversity of srbsdv have not been investigated in the main rice and maize growing areas in china. It is proposed that this virus be considered as a new species, southern rice black streaked dwarf virus, in the group 2 of. The complete genome sequence of two isolates of southern rice blackstreaked dwarf virus, a new member of the genus fijivirus. In recent years, rice production in china has suffered from two emerging viruses, namely, southern rice black streaked dwarf virus srbsdv and rice ragged stunt virus rrsv 2,3. Rice plants infected with this virus exhibit symptoms similar to those caused by rice blackstreaked dwarf virus. It was found that srbsdv infected vectors might contribute negatively to the wbph population, although the longer nymphal period might benefit. Mrdd is caused by the infection of rice black streaked dwarf virus rbsdv and leads to significant economic losses in maize production. To date, control of virus diseases has been based largely on the intensification and development of resistant materials through breeding shimura, 1977.
Further analysis indicated that the apparent alterations in plant growth and morphology were correlated with the expression levels of microrna160, microrna162, microrna167. Southern rice blackstreaked dwarf disease srbsdd was first. Further analysis indicated that the apparent alterations in plant growth and morphology were correlated with the expression levels of microrna160. Methodology open access simultaneous detection and. Rice blackstreaked dwarf virus rbsdv, a member of the genus fijivirus in the family reoviridae, is propagatively transmitted by the small brown planthopper laodelphax striatellus fallen. Emergence of southern rice blackstreaked dwarf virus in. In this study, rice cultivars with high resistance to srbsdv and rrsv were evaluated through field trials in shidian and mangshi county, yunnan province, china. Laboratory of plant virology, south china agricultural university, guangzhou 510642, china. Rice black streaked dwarf virus rbsdv is a plant pathogenic virus of the family reoviridae. In this study, we conducted a genomewide association study gwas on. Quantification of southern rice black streaked dwarf virus and rice black streaked dwarf virus in the organs of their vector and nonvector insect over time. Southern rice blackstreaked dwarf virus srbsdv, a nonenveloped icosahedral virus with a genome of 10 doublestranded rna segments, is a novel species in the genus fijivirus family reoviridae first recognized in 2008.
It was also recently recorded in vietnam 3, 4 and japan. Dot immunobinding assay method with chlorophyll removal for. Rbsdv causes rice blackstreaked dwarf and maize rough dwarf diseases, which lead to severe yield losses in crops in china. Southern rice black streaked dwarf virus srbsdv causes severe harm to rice production. Crop protection volume 41, november 2012, pages 7176. Rice blackstreaked dwarf virus oryza sativa rice 468 172 southern rice black streaked dwarf virus oryza sativa rice 20 876 20 606 sugarcane mosaic virus zea mays maize 100 100 tobacco mosaic virus nicotiana benthamiana tobacco 682 445 tobacco rattle virus nicotiana benthamiana tobacco 142 140. Diseases caused by southern rice black streaked dwarf virus srbsdv and rice ragged stunt virus rrsv considerably decrease grain yield. Southern rice blackstreaked dwarf virus srbsdv directly affects. Rice and maize dwarf diseases caused by the newly introduced southern rice blackstreaked dwarf virus srbsdv have led to severe economic losses in south china in recent years. Southern rice black streaked dwarf virus srbsdv, a new virus from fiji, has seriously damaged rice crops in southern china and northern vietnam in recent years. Southern rice black streaked dwarf virus srbsdv threatens rice production in china and.
Southern rice blackstreaked dwarf virus srbsdv is a recently proposed. A new distribution map is provided for rice black streaked dwarf virus. Southern rice black streaked dwarf virus srbsdv, a genus fijivirus of the family reoviridae, is transmitted by sogatella furcifera whitebacked planthopper, wbph. To date, the p6 protein has been shown to be an rna silencing suppressor and p71 was reported to form tubules in insect cells to facilitate virus spread from the midgut epithelium into the visceral circular muscle through the basal lamina, similar to that reported. Evaluation of rice resistance to southern rice blackstreaked. To date, the p6 protein has been shown to be an rna silencing suppressor and p71 was reported to form tubules in insect cells to facilitate virus spread from the midgut epithelium into the visceral circular muscle through the basal lamina, similar to that reported for southern rice black streaked dwarf virus srbsdv 7,8. Dot immunobinding assay method with chlorophyll removal. The nucleotide sequences of the 10 genomic segments of an italian isolate of maize rough dwarf virus mrdv were determined. This virus is mainly transmitted by the whitebacked planthopper wbph, sogatella furcifera. To well understand the destructive effects of rbsdv infection on maize growth, comparative proteomic analyses of maize. Odorantbinding protein 2 is involved in the preference of. Southern rice blackstreaked dwarf virus srbsdv, recognized as a new member of the genus fijivirus in the family reoviridae was first discovered in guangdong province, china in.
Rice blackstreaked dwarf virus rbsdv and southern rice blackstreaked dwarf virus srbsdv seriously interfered in the production of rice and maize in china. Mar 24, 2014 southern rice blackstreaked dwarf virus srbsdv is a recently discovered member of the genus fijivirus and it is transmitted by the rice whitebacked planthopper wbph, sogatella furcifera horvath. Rice blackstreaked dwarf virus rbsdv is a plant pathogenic virus of the family reoviridae. Since 2009, the virus has rapidly spread and caused serious. Sequence analysis of genome segment s10 of hunan dingcheng isolate of southern rice black streaked dwarf virus genomics and applied biology 2012. Continuing to use this website means you agree to our use of cookies. A novel acetone extract from cottonseed oil sludge was firstly discovered against plant viruses including tobacco mosaic virus tmv, rice stripe virus rsv and southern rice black streaked dwarf virus srbsdv. This study found dufulin dfl, an ideal antisrbsdv molecule, and investigated the interactions of dfl targeting on the nonstructural protein p91. We do not use these to store personal information about you. Here, we focus on the infection of rice by the widespread, devastating virus rbsdv. Although some studies have reported the phylogenetic analysis on rbsdv or srbsdv, the evolutionary. Quantification of southern rice black streaked dwarf virus. The virus disease has caused disastrous damages in rice and maize production in china 1, 2. Although several rbsdv proteins have been studied in detail, the functions of.
The rtqpcr data was analyzed using 7500 software v2. Rice dwarf disease in north vietnam in 2009 is caused by southern rice blackstreaked dwarf virus srbsdv bull inst trop agr kyushu univ. Chemicals that make plants defend themselves could replace. Rice plants infected with this virus exhibit symptoms similar to those caused by rice black streaked dwarf virus. The outbreak of srbsdv in 2009 and 2010 caused serious yield losses in the southern and central region of china and in the northern parts.
Here we attempt to quantify the prevalence of srbsdv in the honghe hani rice terraces system hhrtsa chinese 0yearold traditional rice production system. Jul 15, 2019 the rice black streaked dwarf virus rbsdv disease causes severe rice yield losses in eastern china and other east asian countries. Jian jiang senior research associate consultant fl70 inc. Transgenic rice oryza sativa plants expressing the pns11 protein of rice gall dwarf virus rgdv displayed multiple abnormal phenotypes, some of which were highly reminiscent of the symptoms observed in rgdvinfected rice. Although some studies have reported the phylogenetic analysis on rbsdv or srbsdv, the evolutionary relationship. The disease caused by the virus was firstly observed in yangxi county, guangdong province in 2001, and. A previous study has demonstrated that the host plant preferences of s. Rice blackstreaked dwarf virus p10 suppresses protein kinase.
General information about rice black streaked dwarf virus rbsdv0 this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. The disease caused by the virus was firstly observed in yangxi county, guangdong province in 2001, and then the virus was named by zhou in 2008. Southern rice blackstreaked dwarf virus srbsdv, a new virus from fiji, has seriously damaged rice crops in southern china and northern vietnam in recent years. The virus was also detected by a nested rtpcr assay in corn zea mays, barnyard grass echinochloa crusgalli, juncellus serotinus and flaccidgrass pennisetum flaccidum in andor adjacent to the infected rice fields. Zhousynergism between southern rice blackstreaked dwarf virus and rice ragged stunt virus enhances their insect vector. Southern rice black streaked dwarf virus srbsdv, rice ragged stunt virus rrsv, rice stripe virus rsv, rice grassy stunt virus rgsv, and tomato spotted wilt virus tswv initially infected midgut epithelial cells of their insect vectors kritzman et al. First discovery of acetone extract from cottonseed oil. Thermal inactivation point 10 min of the virus in rice extracts is 60c. It also transmits a virus disease called, southern rice blackstreaked. It was found that srbsdv infected vectors might contribute negatively to the wbph population, although the longer nymphal period might benefit viral acquisition, transmission and increase.
Proteomic analysis of interaction between p71 of southern. Southern rice blackstreaked dwarf virus srbsdv, which causes severe disease symptoms in rice oriza sativa l. Southern rice blackstreaked dwarf virus srbsdv was first identified in yangjiang, guangdong province in china in 2001, and has been proposed as a new member of the genus fijivirus in the family reoviridae. Accumulation of southern rice black streaked dwarf virus srbsdv and rice black streaked dwarf virus rbsdv genome equivalent copies gec in the hemolymph of the vector and nonvector insect species over time using rtqpcr a and conventional rtpcr for. Identification and characterization of micrornas in the. Rice and maize dwarf diseases caused by the newly introduced southern rice blackstreaked. The dwarf disease on rice in hunan in 2009 was the southern rice blackstreaked dwarf disease. This virus is mainly transmitted by the whitebacked planthopper wbph. Complete genomic sequence of maize rough dwarf virus, a. Screening of rice genes interacting with p5b of rice black. Diseases caused by southern rice blackstreaked dwarf virus srbsdv and rice ragged stunt virus rrsv considerably decrease grain yield. The complete genome sequence of two isolates of southern. Transmission characteristics of southern rice blackstreaked dwarf. Southern rice blackstreaked dwarf virus srbsdv is a recently discovered member of the genus fijivirus and it is transmitted by the rice whitebacked planthopper wbph, sogatella furcifera horvath.
Pdf quantification of southern rice black streaked dwarf. Complete genomic sequence of southern rice blackeddwarf. Delphacidae was first found in guangdong province, china. Sep 09, 20 southern rice black streaked dwarf virus srbsdv, a nonenveloped icosahedral virus with a genome of 10 doublestranded rna segments, is a novel species in the genus fijivirus family reoviridae first recognized in 2008. Southern rice black streaked dwarf virus srbsdv was a new pathogen species in genus group fijivirus 2, which caused serious yield loss of many important cereal crops, such as rice and maize, in the past four years. Rice is often subjected to biotic stressors, such as fungi, bacteria, and viruses. Infected rice plants show typical dwarf symptoms, along with dark greening of the leaves, pronounced stunting, twisting of leaf tips, and small white waxy galls along. Comparative proteomic analysis of maize zea mays l. The complete genome sequence of two isolates of southern rice black streaked dwarf virus, a new member of the genus fijivirus. Jian jiang senior research associate consultant fl70. A new proposed fijivirus species in the family reoviridae article pdf available in chinese science bulletin 5323. This information is part of a full datasheet available in the crop protection.
Relation between the viral load accumulation of southern rice. Southern rice blackstreaked dwarf virus srbsdv has spread from the. A novel method for transmitting southern rice blackstreaked dwarf. Southern rice blackstreaked dwarf virus srbsdv is a recently. Close evolutionary relationship between rice blackstreaked. Nov 28, 2008 the virus was also detected by a nested rtpcr assay in corn zea mays, barnyard grass echinochloa crusgalli, juncellus serotinus and flaccidgrass pennisetum flaccidum in andor adjacent to the infected rice fields. In recent years, rice production in china has suffered from two emerging viruses, namely, southern rice blackstreaked dwarf virus srbsdv and rice ragged stunt virus rrsv 2,3. Srbsdv is a novel member of the family reoviridae 4,5 and possesses a genome consisting of 10 doublestranded rna dsrna. Information about rice blackstreaked dwarf virus diagnosis, including distribution. First discovery of acetone extract from cottonseed oil sludge. It was found that srbsdv infected vectors might contribute negatively to the wbph population, although the longer nymphal period might benefit viral acquisition, transmission and.
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